Refining the design of diblock elastin-like polypeptides for self-assembly into nanoparticles

Michèle Dai, Evangelos Georgilis, Guillaume Goudounet, Bertrand Garbay, Jan Pille, Jan C. M. van Hest, Xavier Schultze, Elisabeth Garanger, and Sébastien Lecommandoux

Published: 2021


Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M1V3i]-[I-j]. First, ELP-[M1V3i]-[I-j] (i = 204060j = 2090) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M1V3i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M1V3i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.

Full Access Link: Polymers