Preclinical Feasibility of the Bio-Airbrush for Arthroscopic Cell-Based Cartilage Repair

Koen Dijkstra, Roeland P.J. Huitsing, Roel J.H. Custers, Jan Willem M. Kouwenhoven, Ronald L.A.W. Bleys, Lucienne A. Vonk, and Daniël B.F. Saris

Published: 01/07/2019


Objective: To investigate the preclinical feasibility of using bio-airbrush technology for the fully arthroscopic treatment of cartilage defects with a combination of cells and biomaterials.

Design: A combination of in vitroex vivo, and cadaveric in situ experiments was performed, to evaluate the preclinical feasibility of using bio-airbrush technology for cartilage repair. Cartilage matrix production after airbrushing cells was evaluated. Subsequently, the influence of different spray parameters on successful defect fill was studied. Finally, the surgical feasibility of using bio-airbrush technology was evaluated in situ in cadaveric knees.

Results: The most important findings of the presented study are that the matrix producing capacity of the cells was not hampered by airbrushing, air pressure and defect location were important parameters to consider, and using the bio-airbrush was found to be surgically feasible in a CO2-insufflated arthroscopic procedure in cadaveric knees.

Conclusions: Bio-airbrush using CO2 arthroscopy would facilitate fully arthroscopic application of (cell laden) two-component in situ gelating biomaterials to cartilage defects, as a treatment for cartilage repair. This would shift the conventional procedures, which are commonly performed using a (mini-)arthrotomy, toward arthroscopy.

Full Access Link: Tissue Engineering - Part C: Methods