Optogenetic Methods to Control Tissue Mechanics in Drosophila

Optogenetics is a powerful technique that allows the control of protein function with high spatiotemporal precision using light. Here, we describe the application of this method to control tissue mechanics during Drosophila embryonic development. We detail optogenetic protocols to either increase or decrease cell contractility and analyze the interplay between cell–cell interaction, tissue geometry, and force transmission during gastrulation.